Publikationsliste Elena Kipf

• Originalarbeiten in wissenschaftlichen Fachzeitschriften
• Vorträge
• Konferenzbeiträge

Originalarbeiten in wissenschaftlichen Fachzeitschriften

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2022

• E. Kipf, F. Schlenker, N. Borst, M. Fillies, R. Kirschner-Schwabe, R. Zengerle, C. Eckert, F. von Stetten, M. Lehnert
  Advanced minimal residual disease monitoring for acute lymphoblastic leukemia with multiplex mediator probe PCR
  2022 J. Mol. Diag, Band: 24, Nummer: 1, Seiten: 57 - 68

2021

• F. Schlenker, E. Kipf, N. Borst, N. Paust, R. Zengerle, F. von Stetten, P. Juelg, T. Hutzenlaub
  Centrifugal Microfluidic Integration of 4-Plex ddPCR Demonstrated by the Quantification of Cancer-Associated Point Mutations
  2021 Processes, Band: 2021, Nummer: 9, Ergänzungsband: 97
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  Kurzfassung

  We present the centrifugal microfluidic implementation of a four-plex digital droplet polymerase chain reaction (ddPCR). The platform features 12 identical ddPCR units on a LabDisk cartridge, each capable of generating droplets with a diameter of 82.7 +/- 9 µm. By investigating different oil–surfactant concentrations, we identified a robust process for droplet generation and stabilization. We observed high droplet stability during thermocycling and endpoint fluorescence imaging, as is required for ddPCRs. Furthermore, we introduce an automated process for fourcolor fluorescence imaging using a commercial cell analysis microscope, including a customized software pipeline for ddPCR image evaluation. The applicability of ddPCRs is demonstrated by the quantification of three cancer-associated KRAS point mutations (G12D, G12V and G12A) in a diagnostically relevant wild type DNA background. The four-plex assay showed high sensitivity (3.5–35 mutant DNA copies in 15,000 wild type DNA copies) and linear performance (R2 = 0.99) across all targets in the LabDisk.
• F. Schlenker, E. Kipf, M. Deuter, I. Höffkes, M. Lehnert, R. Zengerle, F von Stetten, F. Scherer, J. Wehrle, N. von Bubnoff, P. Juelg, T. Hutzenlaub, N. Borst
  Stringent Base Specific and Optimization-Free Multiplex Mediator Probe ddPCR for the Quantification of Point Mutations in Circulating Tumor DNA
  2021 cancers, Band: 13, Nummer: 22, Seite: 5742
• P. Juelg, E. Kipf, M. Specht, M. Fillies, C. Eckert, N. Paust, R. Zengerle, M. Lehnert, T. Hutzenlaub
  The MRD disk: automated minimal residual disease monitoring by highly sensitive centrifugal microfluidic multiplex qPCR
  2021 Lab Chip, Band: 21, Seiten: 558 - 570
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  Kurzfassung

  We present a proof-of-principle study on automated, highly sensitive and multiplexed qPCR quantification by centrifugal microfluidics. The MRD disk can be used for standardisation of repetitive, longitudinal assays with high requirements on reproducibility and sensitivity, such as cancer monitoring. In contrast to high-throughput qPCR automation by bulky and expensive robotic workstations we employ a small centrifugal microfluidic instrument, addressing the need of low- to mid-throughput applications. As a potential application we demonstrate automated minimum residual disease (MRD) monitoring of prognostic markers in patients with acute lymphoblastic leukaemia (ALL). The disk-workflow covers all aspects of clinical gold standard MRD quantification: generation of standard curves, specificity controls, no template controls and quantification of the ALL patient sample. We integrated a highly sensitive, colorimetric 2-plex analysis of MRD targets, as well as a 2-plex analysis of reference genes, both in parallel and in a single LabDisk cartridge. For this purpose, a systematic procedure for crosstalk- and signal-to-noise-optimisation is introduced, providing a guideline for efficient multiplex readout inside microfluidic platforms. The qPCR standard curves (n = 12/12) generated on-disk reach clinically required linearity (R2 = 98.1% to R2 = 99.8%). In three consecutive MRD disk runs with an ALL patient sample containing the two representative MRD targets VH3D3D5JH3 and VkIkde, we observe high accordance between the on-disk quantifications (48 ± 6 copies/reaction and 69 ± 6 copies/reaction) and the expected concentrations (57 copies/reaction for both targets). In comparison to the clinical gold standard of manually pipetted, singleplex assays, the MRD disk yields comparable limit of quantification (1 × 10−4) in n = 6/6 analyses (vs. n = 4/4 in gold standard) and a limit of detection (1 × 10−5) in n = 6/6 analysis (vs. n = 2/4 in gold standard). The automation reduces the risk of manual liquid handling errors, making the MRD disk an attractive solution to assure reproducibility in moderate-throughput, longitudinal gene quantification applications.
• F. Schlenker, E. Kipf, N. Borst, T. Hutzenlaub, R. Zengerle, F. von Stetten, P. Juelg
  Virtual Fluorescence Color Channels by Selective Photobleaching in Digital PCR Applied to the Quantification of KRAS Point Mutations
  2021 Analytical Chemistry, Band: 93, Nummer: 30, Seiten: 10538 - 10545
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  Kurzfassung

  Multiplexing of analyses is essential to reduce sample and reagent consumption in applications with large target panels. In applications such as cancer diagnostics, the required degree of multiplexing often exceeds the number of available fluorescence channels in polymerase chain reaction (PCR) devices. The combination of photobleaching-sensitive and photobleaching-resistant fluorophores of the same color can boost the degree of multiplexing by a factor of 2 per channel. The only additional hardware required to create virtual fluorescence color channels is a low-cost light-emitting diode (LED) setup for selective photobleaching. Here, we present an assay concept for fluorescence color multiplexing in up to 10 channels (five standard channels plus five virtual channels) using the mediator probe PCR with universal reporter (UR) fluorogenic oligonucleotides. We evaluate the photobleaching characteristic of 21 URs, which cover the whole spectral range from blue to crimson. This comprehensive UR data set is employed to demonstrate the use of three virtual channels in addition to the three standard channels of a commercial dPCR device (blue, green, and red) targeting cancer-associated point mutations (KRAS G12D and G12V). Moreover, a LOD (limit of detection) analysis of this assay confirms the high sensitivity of the multiplexing method (KRAS G12D: 16 DNA copies/reaction in the standard red channel and KRAS G12V: nine DNA copies/reaction in the virtual red channel). Based on the presented data set, optimal fluorogenic reporter combinations can be easily selected for the application-specific creation of virtual channels, enabling a high degree of multiplexing at low optical and technical effort.

2019

• P. Juelg, M. Specht, E. Kipf, M. Lehnert, C. Eckert, M. Keller, T. Hutzenlaub, F. von Stetten, R. Zengerle, N. Paust
  Automated serial dilutions for high-dynamic-range assays enabled by fill-level-coupled valving in centrifugal microfluidics
  2019 Lab Chip, Band: 19, Seiten: 2205 - 2219
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  Kurzfassung

  We introduce a new concept for centrifugal microfluidics that enables fully automated serial dilution generation without any additional means besides temperature control. Key feature is time-independent, serial valving of mixing chambers by fill-level-coupled temperature change rate (FLC-TCR) actuated valving. The automated dilution is realized under continuous rotation which enables reliable control of wetting liquids without the need of any additional fabrication steps such as hydrophobic coatings. All fluidic features are implemented in a monolithic fashion and disks are manufactured by foil thermoforming for scalable manufacturing. The new valving concept is demonstrated to reliably prevent valving if the diluted sample is not added to the mixing chamber (n = 30) and ensures valving if the dilution stage is completed (n = 15). Accuracy and precision of the automated serial dilution are verified by on-disk generation of qPCR standard curve dilutions and compared with manually generated reference dilutions. In a first step, the 5-log-stages standard curves are evaluated in a commercial qPCR thermocycler revealing a linearity of R² ≥ 99.92 % for the proposed LabDisk method vs. R² ≥ 99.67 % in manual reference dilutions. In a second step, the disk automated serial dilution is combined with on-disk qPCR thermocycling and readout, both inside a LabDisk Player. A 4-log-stages linearity of R² ≥ 99.81 % and a sensitivity of one leukemia associated ETV6-RUNX1 mutant DNA copy in a background of 100,000 wild-type DNA copies is achieved.

2018

• E. Kipf, S. Sané, D. Morse, T. Messinger, R. Zengerle, S. Kerzenmacher
  An air-breathing enzymatic cathode with extended lifetime by continuous laccase supply
  2018 Bioresource Technol, Band: 264, Seiten: 306 - 310
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  Kurzfassung

  We present a novel concept of an air-breathing enzymatic biofuel cell cathode combined with continuous supply of unpurified laccase-containing supernatant of the white-rot fungus Trametes versicolor for extended lifetime. The air-breathing cathode design obviates the need for energy-intensive active aeration. In a corresponding longterm experiment at a constant current density of 50 μA cm−2, we demonstrated an increased lifetime of 33 days (cathode potential above 0.430 V vs. SCE), independent of enzyme degradation. The obtained data suggest that theoretically a longer lifetime is feasible. However, further engineering efforts are required to prevent clogging and fouling of the supply tubes. These results represent an important step towards the realization of enzymatic biofuel cell cathodes with extended lifetime and enhanced performance.
• M. Lehnert, E. Kipf, F. Schlenker, N. Borst, R. Zengerle, F. von Stetten
  Fluorescence signal-to-noise optimisation for real-time PCR using universal reporter oligonucleotides
  2018 Anal Methods-uk
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  Kurzfassung

  In this study we optimised the fluorescence signal generation of contact quenched universal reporter oligonucleotides. These are used as secondary probes in real-time Mediator Probe PCR to detect the sequence-specific cleavage of label-free primary mediator probes. Since the fluorescence signal generation of a universal reporter is not influenced by the target DNA sequence, optimisation of the fluorescence signal-to-noise ratio will improve the performance of all Mediator Probe PCRs that are based on this type of universal reporter. To determine the critical factors influencing signal-to-noise optimisation, we systematically analysed four parameters. These parameters were type of fluorophore, type of quencher molecule, intramolecular orientation of both residuals, and the number of quencher labels. In total, more than 30 different fluorogenic universal reporter structures were analysed, covering the whole fluorescence spectrum from green to crimson. From our results, we deduced a novel set of guidelines for signal-to-noise optimisation in the design of contact quenched, fluorogenic universal reporter oligonucleotides. We confirmed these guidelines in a different thermocycler, and by designing a second set of universal reporters, which were used for multiplex real-time PCR quantification of acute lymphoblastic leukaemia marker sequences. This optimised biplex Mediator Probe PCR showed an improved performance under clinical conditions, with a 10 times higher resolution regarding the limit of quantification. In addition to Mediator Probe PCR, these guidelines may also prove useful in signal-to-noise optimisation of other fluorescence-based assays where contact quenched oligonucleotides or secondary reporter molecules are used.
• E. Kipf, J. Erben, R. Zengerle, J. Gescher, S. Kerzenmacher
  Systematic investigation of anode materials for microbial fuel cells with the model organism G. sulfurreducens
  2018 Bioresource Technology Reports, Band: 2, Seiten: 29 - 37
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  Kurzfassung

  Different carbon and metal-based anode materials for microbial fuel cells were systematically investigated with a pure culture of the model organism G. sulfurreducens. The highest limiting current density of 756 ± 15 μA cm-2 at -0.253 ± 0.037 V vs. SCE was achieved with graphite foil using a step-wise galvanostatic technique. But also the application of completely different anode materials such as activated carbon cloth, stainless steel and graphite felt led to similar high limiting current densities, suggesting that G. sulfurreducens is able to use a large range of substantially different anode materials as external electron acceptor. Additionally, we could show that a step-wise galvanostatic technique to record polarization curves yields similar current densities as potentiostatic control at -0.400 V vs. SCE with the investigated carbon-based materials. In case of stainless steel these techniques yield slightly different results, presumably due to an effect related to the material’s surface properties.

2014

• E. Kipf, R. Zengerle, J. Gescher, S. Kerzenmacher
  How Does the Choice of Anode Material Influence Electrical Performance? A Comparison of Two Microbial Fuel Cell Model Organisms
  2014 ChemElectroChem, Band: 1, Seiten: 1849 - 1853
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  Kurzfassung

  The influence of the anode material on the electrical performance of the two microbial fuel cell model organisms Geobacter sulfurreducens and Shewanella oneidensis is investigated. High-surface-area activated carbon and low-surface-area graphite felt are compared in terms of polarization curves under quasi-steady-state conditions. Unexpectedly, G. sulfurreducens exhibits similar current densities up to 700 mAcm_2 independent of the anode material. This is ~50% higher than steady-state values reported previously. The negligible influence of electrode material on the electrical performance of G. sulfurreducens is attributed to the fact that it performs only direct electron transfer, but forms thick biofilms. In contrast, S. oneidensis, relying mainly on mediated electron transfer, apparently makes better use of high-surface-area activated carbon and achieves higher current densities compared to graphite felt. This underlines the importance of tailoring anode materials according to the used organisms.

2013

• E. Kipf, J. Koch, B. Geiger, J. Erben, K. Richter, J. Gescher, R. Zengerle, S. Kerzenmacher
  Systematic screening of carbon-based anode materials for microbial fuel cells with Shewanella oneidensis MR-1
  2013 Bioresource Technol, Band: 146, Seiten: 386 - 329
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  Kurzfassung

  We present a systematic screening of carbon-based anode materials for microbial fuel cells with Shewanella oneidensis MR-1. Under anoxic conditions nanoporous activated carbon cloth is a superior anode material in terms of current density normalized to the projected anode area and anode volume (24.0 ± 0.3 μA cm−2 and 482 ± 7 μA cm−3 at −0.2 vs. SCE, respectively). The good performance can be attributed to the high specific surface area of the material, which is available for mediated electron transfer through self-secreted flavins. Under aerated conditions no influence of the specific surface area is observed, which we attribute to a shift from primary indirect electron transfer by mediators to direct electron transfer via adherent cells. Furthermore, we show that an aerated initial growth phase enhances the current density under subsequent anoxic conditions fivefold when compared to a similar experiment that was conducted under permanently anoxic conditions.

Vorträge

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2024

• E. Kipf, P. Jülg, F. Schlenker, M. Fillies, C. Kranig, M. Specht, S. Groeneveld-Krentz, S. Neumann, R. Zengerle, F. von Stetten, N. Borst, R. Kirschner-Schwabe, T. Hutzenlaub, M. Lehnert, C. Eckert
  Messung des individuellen Therapieansprechens von akuten lymphoblastischen Leukämien (IRMA-4-ALL)
  2024 Translationale Forschung in der Personalisierten Medizin – Chancen und Hürden, Netzwerkveranstaltung für geförderte Projekte der „Innovationen für die individualisierte Medizin“ und der „Translationsp

Konferenzbeiträge

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2024

• T-T. Truong, Y. Kaku, J. Schlanderer, F. Schlenker, E. Kipf, E. Dazert, F. Axt, N. von Bubnoff, S. Kartmann, N. Borst, N. Paust, T. Hutzenlaub, P Juelg
  A robotic microfluidic approach for rapid sample-to-answer analysis of cell-free DNA
  2024 13th CNAPS International Symposium on Circulating Nucleic Acids in Plasma and Serum, Graz/Austria, 4-6 March 2024

2021

• P. Juelg, E. Kipf, M. Specht, J. Menges, M. Fillies, C. Eckert, N. Paust, R. Zengerle, M. Lehnert, T. Hutzenlaub
  Die MRD Disk: Automatisiertes Monitoring Minimaler Resterkrankung durch hochsensitive, zentrifugal-mikrofluidische Multiplex-qPCR / The MRD disk: automated minimal residual disease monitoring by highly sensitive centrifugal microfluidic multiplex qPCR
  2021 MST

2020

• F. Schlenker, E. Kipf, N. Borst, T. Hutzenlaub, N. Paust, R. Zengerle, F. von Stetten, P. Juelg
  Centrifugal microfluidic 4 Plex digital droplet PCR for quantification of circulating tumor DNA
  2020 MicroTAS 2020, 04.-09.10.2020, virtual
• F. Schlenker, E. Kipf, M. Deuter, I. Hoeffkes, N. Borst, T. Hutzenlaub, R. Zengerle, F. von Stetten, N. von Bubnoff, P. Juelg
  Low optimization effort for highly sensitive assays for point mutation quantification in CTDNA: the Mediator Probe digital PCR
  2020 Liquid Biopsy Symposium, 30.10.2020, virtuell

2019

• M. Lehnert, E. Kipf, F. Schlenker, R. Zengerle, N. Borst, F. von Stetten
  Multiplex Mediator Probe Real-Time PCR - Optimization and Guideline Development through Systematic Characterisation of Label Free Mediator Probes and Fluorogenic Universal Reporters
  2019 9th Gene Quantification Event qPCR dPCR & NGS 2019, 18 - 22 March 2019, in Freising-Weihenstephan, Germany
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  Kurzfassung

  Mediator Probe PCR is a powerful and robust real-time PCR technology for multiplex DNA detection and quantification. It uses label free mediator probes, for molecular detection of nucleic acids during DNA amplification, in combination with fluorogenic universal reporters for signal generation. During PCR, target sequence specific mediator probes are cleaved by the polymerase and a generic sequence, the mediator, is set free. In the second step the mediator binds to the universal reporter, where it is extended by the polymerase. This generates a strong fluorescence signal increase. Due to the separation of DNA detection and signal generation many advantages arise. Mediator probes are not limited in their design by properties of the target sequence and a standard set of highly optimised fluorogenic universal reporters can be used for multiplex Mediator Probe PCR, right from the start.1 In the last years Mediator Probe PCR evolved from an innovative new method to an optimised and robust multiplexing technology. This was achieved by systematic characterisation of its molecular processes, which again was advantaged by the separation of DNA detection and signal generation. A DoE approach was used for the optimisation of Mediator Probes, focusing on their binding strengths.2 In parallel, a set of universal reporters with improved signal-to-noise ratios was established by successive testing over 40 molecular structures with different fluorophore-quencher labels and configurations.1 As a result, distinct guidelines exist, which enable fast adaption of new DNA targets and facilitate multiplex Mediator Probe PCR design. The capability of the technology was shown by highly sensitive, precise and specific multiplex Mediator Probe real-time PCRs in different areas of molecular diagnostics. These fields include monitoring of oncological disease, detection of pathogens or analysis of food samples.1,3
• F. Schlenker, E. Kipf, S. Jenne, N. Borst, J. Lüddecke, K. Dormanns, T. Hutzenlaub, J. Steinert, N. Paust, R. Zengerle, F. von Stetten, P. Juelg
  Doubling the order of color-multiplexing by photobleaching in Mediator Probe droplet digital PCR
  2019 EACR-ESMO Joint Conference on Liquid Biopsies, Bergamo/Italien, 15. – 17. 05. 2019
• P. Juelg, M. Specht, M. Meyer, E. Kipf, F. Schlenker, F. Baensch, S. Neumann, F. von Stetten, R. Zengerle, N. Paust, M. Fillies, R. Kirschner-Schwabe, S. Groeneveld-Krentz, M. Lehnert, C. Eckert, T. Hutzenlaub
  Individual Response Monitoring Assay (IRMA) – Standardization Of Personalized Multiplex Biomarker Quantification
  2019 EACR-ESMO Joint Conference on Liquid Biopsies, Bergamo/Italien, 15. – 17.05. 2019
• P. Juelg, M. Specht, M. Meyer, E. Kipf, F. Schlenker, F. Bänsch, S. Neumann, F. von Stetten, R. Zengerle, N. Paust, M. Fillies, R. Kirschner-Schwabe, S. Groeneveld-Krentz, M. Lehnert, C. Eckert, T. Hutzenlaub
  Standardization of Personalized Multiplex Biomarker Quantification: Individual Response Monitoring Assay (IRMA)
  2019 MST-Kongress, 28. - 30.Oktober 2019, Berlin

2018

• P. Juelg, M. Specht, E. Kipf, M. Lehnert, C. Eckert, N. Paust, F. von Stetten, R. Zengerle, T. Hutzenlaub
  Automation of qPCR based Minimal Residual Disease Monitoring by Centrifugal Microfluidics
  2018 11th Symposium on minimal residual cancer, 03. – 05.05.2018, Montpellier, Frankreich
• P. Juelg, M. Specht, E. Kipf, M. Lehnert, C. Eckert, N. Paust, R. Zengerle, T. Hutzenlaub
  LabDisk for fully automated quantification of two leukemia associated gene targets
  2018 MicroTAS 2018, 11. -15. November 2018, Kaohsiung / Taiwan
• P. Juelg, M. Specht, E. Kipf, M. Lehnert, C. Eckert, N. Paust, F. von Stetten, R. Zengerle, T. Hutzenlaub
  Towards Standardization of Molecular Diagnostic Workflows: Centrifugal Microfluidic Automation of qPCR for Cancer Monitoring
  2018 6th International Molecular Diagnostics Europe, Lissabon / Portugal, 22. - 24. 05.2018

2017

• P. Juelg, M. Specht, E. Kipf, M. Lehnert, C. Eckert, S. Wadle, M. Keller, N. Paust, R. Zengerle, T. Hutzenlaub
  Centrifugal microfluidic automation of dilution series for high dynamic range assays demonstrated for decadal dilutions up to 1:100,000
  2017 MicroTAS 2017, Savannah /USA, 22.-26.10.2017
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  Kurzfassung

  For the first time we present the centrifugal microfluidic automation of an extensive dilution series that can cover all biochemically relevant dilution ranges. In contrast to existing methods, the new concept is based on iteration loops of individual dilution steps that can be easily combined in a theoretically unlimited fashion. Thus, any combination and range of dilutions can be implemented. The concept was demonstrated by generating a qPCR standard curve for cancer biomarker ETV6-RUNX1 up to 1:100,000. We achieved the following analytical performance characteristics: A high coefficient of determination of R²=98.9 %, equidistant quantification cycles of ΔCq=3.4±0.3 and low replicate variation coefficients of CV<3.5 %.

2015

• E. Kipf, D. Morse, T. Messinger, S. Sané, S. Kerzenmacher
  An air-breathing enzymatic cathode with extended lifetime by continuous Laccase supply
  2015 European Fuel Cell Technology & Applications Conference - Piero Lunghi Conference December 16-18, 2015, Naples, Italy

2014

• E. Kipf, T. Messinge, S. Sané, S. Kerzenmacher
  An air-breathing cathode based on buckypaper electrodes with reversibly adsorbed laccase
  2014 2nd European International Society for Microbial Electrochemistry and Technology Meeting, Alcala, Spain, 03.-05-09.2014
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  Kurzfassung

  Previously, we presented buckypaper electrodes with reversibly adsorbed laccase as promising cathodes for biofuel cell applications [1]. This concept is particularly advantageous, since the periodic exchange of the enzyme solution against fresh enzymes can help to overcome the limited lifetime caused by denaturation of the enzymatic biocatalyst. Here, we present the development of a corresponding air-breathing cathode, which enables passive operation without energy-intensive aeration of the catholyte. Thereto, buckypaper electrodes [2] were equipped with a 0.5 mm thin silicone membrane as diffusion layer which enables sufficient oxygen supply but prevents salt crust formation and electrolyte leakage [3]. As catholyte 0.1 M Na-acetate buffer (pH 5) containing 3.6 U/mL laccase from T. versicolor (Sigma-Aldrich, Germany) was used in two different configurations. The setup with 20 mL catholyte volume was operated in batch mode, similar to previous experiments with active aeration. In addition, a thin flat-plate configuration with minimized ohmic resistance and a small catholyte volume of only 0.29 mL was investigated in batch and continuous mode. The batch-type air-breathing cathode with 20 mL volume yielded ~78 µA/cm² at 0.4 V vs. SCE, which is in the same range as corresponding electrodes in actively aerated laccase solution [1]. In comparison, the flat-plate cathode with smaller catholyte volume yielded only ~12 µA/cm² in batch-mode. This indicates that not enough laccase was supplied to the electrode to sustain higher current densities. However, by applying a continuous laccase flow through the catholyte chamber (HRT: 3.5h), the current density could be increased to ~53 µA/cm2 at 0.4 V vs. SCE. This underlines that the performance is governed by the amount of enzymes available for adsorption. In the next step, optimum flow-rates for the advantageous flat-plate configuration will be identified, and the long-term stability of the air-breathing cathode with continuous supply of fresh laccase will be investigated.

2013

• E. Kipf, D. Thissen, S. Kerzenmacher
  Air-breathing cathodes for microbial fuel cells based on activated carbon cloth and spin-coated PDMS membranes as hydrophobic diffusion layer
  2013 4th International Microbial Fuel Cell Conference, Cairns, Australia, 01. – 04.09.2013 , Seiten: 58 - 59
• J. Erben, A. Sido, E. Kipf, S. Thiele, S. Kerzenmacher
  Improving current production of Shewanella oneidensis MR-1 with electrospun anode materials
  2013 4th International Microbial Fuel Cell Conference, Cairns, Australia, 01.09.2013 – 04.09.2013 , Seiten: 188 - 189
• S. Kerzenmacher, J. Danzer, E. Kipf, A. Kloke, C. Köhler, S. Sané, R. Zengerle
  Neue Materialien und Konzepte für Biobrennstoffzellen
  2013 Microsystemtechnik (MST) Kongress 2013, Aachen, 14. - 16.10.2013 , Seiten: 626 - 629
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  Kurzfassung

  Biobrennstoffzellen sind ein vielversprechender Ansatz zur autarken Energieversorgung verteilter Systeme, wie z.B. medizinische Implantate oder verteilte Sensoren. In unserem Beitrag stellen wir neue Materialien und Konzepte vor, um die Leistung und Langzeitstabilität dieser Systeme zu verbessern.

2012

• E. Kipf, J. Koch, B. Geiger, S. Kerzenmacher
  Systematic comparison of anode materials for microbial fuel cells
  2012 7. EU-ISMET 2012 in Ghent, Belgium, 27.09. – 28.09.2012 Comm. Appl. Biol. Sci, Ghent University, 77/2, 2012, Seite: 128

2011

• S. Kerzenmacher, J. Danzer, E. Kipf, A. Kloke, C. Köhler, S. Rubenwolf, S. Sané, R. Zengerle, J. Gescher
  Biobrennstoffzellen zur Energieversorgung von Mikrosystemen: vom energieautarken Implantat hin zum dezentralen Umwelt-Monitoring
  2011 Mikrosystemtechnik-Kongress, Darmstadt, Deutschland, October 10-12 , Seiten: 375 - 378
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  Kurzfassung

  Biobrennstoffzellen sind ein vielversprechender „Energy Harvesting“-Ansatz, bei dem die chemische Energie eines in der Umgebung verfügbaren Substrats in direkt nutzbare Elektrizität umgewandelt wird. In unserem Beitrag werden die verschiedenen Biobrennstoffzellenkonzepte vorgestellt und die zukünftigen Herausforderungen und Chancen der Tech-nologie an Hand von typischen Anwendungsbeispielen diskutiert.
• E. Kipf, J. Koch, S. Kerzenmacher
  Systematic comparison of anode materials for microbial fuel cells
  2011 Marine Resources and Beyond, Bremerhaven, Germany, September 5-7 , Seite: 95
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  Kurzfassung

  We present a systematic comparison of anode materials for microbial fuel cells (MFCs). Therein the anodes are assembled as half-cells and galvanostatic polarization curves are recorded against a reference electrode. This approach led to the identification of activated carbon cloth (ACC) as superior anode material compared to graphite felt (GF), which at present is predominantly used in microbial fuel cells.