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Single Bacteria Genomics and MALDI-TOF

Projektbild

Project description

Cultivation is the gold standard for pathogen identification in medical microbiology. However, it is a time consuming method which often prevents rapid and precise diagnosis of infectious diseases. Moreover, the vast majority of bacterial species found in clinical samples or the environment is considered to be uncultivable in the lab. Single-cell analysis allows for molecular characterization of individual bacteria and thereby bypasses the cultivation step. Thereby, new species and genes from even uncultivable bacteria can be identified and further this strategy offers the potential for rapid diagnosis of infectious diseases. In SiBaGem we will utilize the Single-Cell Printer (SCP) technology to develop highly automated assays for genomic and transcriptomic analysis of individual bacterial cells. Further, we will investigate the generation of picoliter droplets which is essential for the SCP technology: In detail we will look at (i) the electrostatic neutralization of droplets, (ii) a method for high throughput droplet sorting, and (iii) the controlled co-crystallization of a MALDI matrix for subsequent single-cell typing with a novel mass spectrometer. The goal of the SiBaGeM consortium is to develop a laboratory device for single-cell genomics and a single-cell mass spectrometer for rapid diagnosis of infectious diseases.

Start/End of project

01.06.2016 until 30.11.2018

Project manager

Julian Riba (Prof. Dr. Roland Zengerle)

Contact person

Julian Riba
Phone:0761 203-73216

Partners

Cytena GmbH, Freiburg Biosparq B.V., Amsterdam Medical Center

Funding

BMBF - Eurostars Programm

Keywords

InkJet & Drucktechnologien, Analytik & Diagnostik, Mikrodosierung, Einzelzellanalyse, Genomanalyse, Sequenzierung, Massenspektroskopie, Medizinische Mikrobiologie, inkjet & printing technologies, analytics & diagnostics, microdosing, single-cell analysis, single-cell genomics, sequencing, mass spectrometry, medical microbiology
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